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After excitation of terbium fluorescence at 337 nm, emission at 490 nm (terbium) and 520 nm (GFP) were recorded with a 70-μs delay over 600 μs to reduce background fluorescence, and the reaction was followed over 60 cycles of each data point using a PHERAstar FS microplate reader (BMG Labtech)

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Microbial secondary metabolites via fermentation approaches for dietary supplementation formulations